Mouse Anti-MUC1 Recombinant Antibody (TAB-418MZ)

Customer Review

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David Walker
09.Sep,23

Great for MUC1 Detection

The Anti-MUC1 Recombinant Antibody has been excellent for our MUC1 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

Emily Turner
11.Oct,22

Reliable in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for MUC1 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

John Harris
20.Jul,21

Consistent and High-Quality

This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for MUC1 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Q&As

1. Is the anti-MUC1 recombinant antibody suitable for use in Western blotting?

   A: Yes, the anti-MUC1 recombinant antibody (TAB-418MZ) is suitable for use in Western blotting. It provides specific binding to MUC1, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-MUC1 recombinant antibody?

   A: The recommended storage condition for the anti-MUC1 recombinant antibody (TAB-418MZ) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-MUC1 recombinant antibody be used in immunoprecipitation assays?

   A: Yes, the anti-MUC1 recombinant antibody (TAB-418MZ) can be used in immunoprecipitation assays. It provides specific binding to MUC1, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-MUC1 recombinant antibody effective in ELISA applications?

   A: Yes, the anti-MUC1 recombinant antibody (TAB-418MZ) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of MUC1.

5. What is the optimal dilution for using the anti-MUC1 recombinant antibody in immunofluorescence?

   A: The optimal dilution for using the anti-MUC1 recombinant antibody (TAB-418MZ) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Kvorjak, Michael, et al. "Cross-talk between colon cells and macrophages increases ST6GALNAC1 and MUC1-sTn expression in ulcerative colitis and colitis-associated colon cancer." Cancer immunology research 8.2 (2020): 167-178. https://doi.org/10.1158/2326-6066.cir-19-0514
   The study investigated the interaction between colon cells and macrophages in the context of ulcerative colitis (UC) and colitis-associated colon cancer (CACC). Researchers found that macrophages, particularly the M2-like phenotype, play a significant role in promoting the expression of the glycosyltransferase ST6GALNAC1 in colon cancer cells. This enzyme catalyzes the addition of sialic acid to O-linked GalNAc residues, leading to the formation of tumor-associated sialyl-Tn (sTn) antigens on the mucin MUC1. The study demonstrated that the presence of M2 macrophages, through cytokines such as IL13 and CCL17, increased the expression of ST6GALNAC1 and the corresponding MUC1-sTn glycoform, contributing to the pathogenesis and progression of UC and CACC.
   Creative Biolabs provided the anti-MUC1 antibody 5E5 (Cat#: TAB-418MZ) used in the study. This antibody was crucial for identifying and quantifying the MUC1-sTn glycoform in tissue samples and cell cultures. By enabling the detection of specific glycoforms of MUC1, the antibody facilitated the evaluation of macrophage-induced changes in glycosylation patterns, thus playing a vital role in elucidating the mechanisms through which macrophages influence colon cancer progression.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-418MZ, RRID: AB_2941837)

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Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Kvorjak, Michael, et al. "Crosstalk between colon cells and macrophages increases ST6GALNAC1 and MUC1-sTn expression in ulcerative colitis and colitis-associated colon cancer." Cancer Immunology Research (2019).

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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